Application

cell adhesion, pharmacology, vascular therapy research, coagulation, drug discovery, regulation of vascular tone

Cell Line Origin

Vein

Components

Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

General description

HUVSMC has been used in studies evaluating treatments that involve blood vessel stents. Specifically, intra-shunt delivery of motexafin gadolinium was shown to inhibit the proliferation of SMCs during treatment of portal hypertension (Wang, 2013). Also, high-density lipoproteins (HDL) exhibit cardioprotective and anti-inflammatory properties by reducing HUVSMC expression of chemokines CCL2, CCL5, and CX3CL1, and chemokine receptors CCR2 and CX3CR1 (via NF-κB/pAkt inhibition) and by decreasing cell proliferation (via pERK inhibition), highlighting HDL importance in preventing neointimal hyperplasia, the main cause of early vein graft/stent failure (van der Vorst, 2013). HUVSMC were also used to demonstrate that genes coding for muscle and non-muscle actin proteins are transcribed by different transcription machinery (Lee, 2011).Characterization: positive for smooth muscle cell specific alpha-actin expression.

Preparation Note

2nd passage, >500,000 cells in Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 6 doublings

Subculture Routine

Please refer to the HUVSMC Culture Protocol.